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Cellular decline in NAD + is associated with impairment of mitochondrial function associated with ageing, and pharmacologically increasing NAD + levels in old mice restores mitochondrial function comparable to that in young mice in a SIRT1-dependent manner 66,67

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For instance, succinate at a final concentration of 5 mM can induce a substantial increase in O 2 • − production from the FMN site of Complex I (Pryde and Hirst, 2011)