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coli cells carrying the appropriate plasmid were grown in YT-broth supplemented with 50 µg/ml ampicillin (Merck, Darmstadt, Germany) to an OD 600 of 0.5

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In the same study, Lactobacilli were determined to be the most abundant in dam-reared pups, present in pups supplemented with MFGM, but not detected in pups fed control formula (152)