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After treatment, the PC12 cells were added with 100 μM cycloheximide to interfere with the translocation step in protein synthesis

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This explains why in anorexia nervosa there is an increase in plasma ghrelin concentrations, while in obese subjects it is reduced, and also why GH secretion is increased in anorexia nervosa patients and during fasting, while it is reduced or absent in obesity, but also in the elderly [170] (Figure 12A)