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② Preparation of Standard Solutions Accurately weigh an appropriate amount of glutathione standard, dissolve it in a volumetric flask with deionized water, mix well, dilute to volume, and prepare a series of standard solutions with concentrations of 25 μg/mL, 50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL, and 800 μg/mL