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For HY-switch mice, cells were stained with AQUA Zombie live/dead dye (BioLegend #423101) according to the manufacturer’s instruction and then surface stained for TCRβ (BioLegend #2629564), CD8α (BioLegend #2562610), CD4 (BioLegend #893330), TCR H-Y (eBioScience #466267), CD3ε (eBioScience #469315), NK1.1 (BioLegend #313312), CD19 (BioLegend #313641) and CD25 (BioLegend #313392) in the presence of an Fc receptor blocking antibody (BioLegend AB_1574973) during 30 min at 4 °C
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