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Disrupted feeding times – such as eating during the biological night or at irregular schedules – can themselves induce circadian misalignment and worsen sleep quality

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Twenty male and female Sprague-Dawley rats arrived at six weeks of age and were housed singly (socially isolated, SI) or in groups of 2–3 per cage (group housed, GH) for six weeks from six weeks to twelve weeks of age on a 12 h reverse light-dark cycle (lights on at 9:00pm) with access to standard rat chow and water ad libitum