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The ipsilateral hippocampus was isolated and homogenized with Radio Immunoprecipitation Assay (RIPA) lysate containing phosphatase inhibitor, protein phosphatase inhibitor and Phenylmethylsulfonyl fluoride (PMSF) (1:100) in a homogenizer at 4°C to thoroughly cleave the proteins, and then centrifuged to collect the supernatant
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Type:78g
Zhou S, Wang L, Li G, Liu R
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