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Membranes were washed with PBS/0.1% saponin and incubated with secondary antibodies DAPI (Thermo Fisher Scientific), donkey-anti-mouse-AF647 (Thermo Fisher Scientific), donkey-anti-rabbit-Cy3 (Jackson ImmunoResearch, Cambridgeshire, UK) and donkey-anti-goat-AF488 (Thermo Fisher Scientific) for 1 h at RT
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