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The full-length human eEF1A1 cDNA was amplified using pOTB7-eEF1A1 as a template and two oligonucleotides 5′-CG GAATTC CG GCGGCCGC TATGGGAAAGGAAAAGACTCATATCAACATTG-3′ (sense) and 5′-GG GGTACC TCATTTAGCCTTCTGAGCTTTCTGGGCAG-3′ (antisense), where the underlined nucleotides specify the EcoRI, NotI and KpnI sites, respectively
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Three included studies in our review investigated age-specific alterations in GSH levels in autopsy brain tissue from healthy, cognitively unimpaired adults, using HPLC (Tong et al., 2016) or colorimetry (Harish et al., 2012, Venkateshappa et al., 2012)
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