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In this group, CCL3 and IL1B were the most responsive genes, at the transcriptional level, to B12, but no differences were observed in the intracellular levels of the respective proteins ( Figures 3E, F )

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Treatment is contraindicated during use and for 6 months after stopping Platelet disorders or anticoagulant therapy — conditions affecting platelet function or blood clotting require medical review before PRP treatment Active eczema, psoriasis, or dermatitis in the treatment area — microneedling over inflamed skin can exacerbate these conditions Keloidal scarring tendency — patients who form keloid scars require careful assessment, as microneedling creates controlled skin injury Pregnancy or breastfeeding — as a precautionary measure, treatment is deferred until after breastfeeding is complete Active malignancy — growth factors may theoretically stimulate cancerous cell activity Recent sunburn or chemical peel — the skin must be intact and non-compromised before microneedling Clinical Disclaimer PRP facial with microneedling is a biological medical procedure