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Cells were resuspended in a Low Salt Lysis Buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM PMSF, and 3 mM 2-mercaptoethanol), lysed by sonication, and the soluble fraction isolated by centrifugation and incubated with low-density nickel agarose beads (GoldBio)
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