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Subsequent experiments that used Cystector and the H 2 O 2 biosensor HyPer7 in parallel wells (Pak et al., 2020) showed an immediate response of HyPer7 upon the H 2 O 2 treatment, indicating that H 2 O 2 -induced disulfide formation precedes intracellular cysteine depletion (Fig S3)
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